GENE POLYMORPHISM OF TRANSFORMING GROWTH FACTOR BETA-1 (TGF-Β1) AND ITS LEVEL IN RHEUMATOID ARTHRITIS PATIENTS ASSOCIATED WITH OSTEOPOROSIS DISEASE

Abstract

Objective: The imbalance between pro-inflammatory and anti-inflammatory cytokines is a feature of rheumatoid arthritis (RA). The role of transforming growth factor beta-1 (TGF- β1) and its receptor in the pathogenesis of RA is conflicting. We aim to investigate the role of polymorphisms in the gene in susceptibility and severity of RA and osteoporosis.
Methods: Eighty-six RA patients and eighty-four control were enrolled in the study.
Genotyping of TGF- β1 T/C869 and TGF- β1 G/A800 was determined by restriction fragment length polymorphism-polymerase chain reaction (PCR-RFLP).
Results: Subjects carrying T allele of TGF- β1 T/C869 genotype were significantly more likely to have osteoporosis than subjects carrying C allele (OR:6.95, 95% CI: 2.7-15.7, P: 0.001).Also, Subjects with A allele of TGF- β1 G/A800 genotype were significantly more likely to have osteoporosis (OR: 1.13, 95% CI: 1.05-1.2, P: 0.001).
Conclusion: T/C869 genotype and G/A800 genotype of TGF-β1 gene affecting on severity of RA and have the ability to develop the risk to osteoporosis in patients carrying T allele and A allele of T/C869 and G/A800 gene polymorphism respectively
Keywords: Transforming growth factor beta-1 (TGF-β1), Rheumatoid arthritis, Polymorphism, Osteoporosis