BLEOMYCIN INDUCED –INTERSTITIAL PULMONARY FIBROSIS IN MICE

Abstract

The uses of animal models in researches of pulmonary fibrosis depend on the presence of structural, biochemical, and molecular similarity between fibrotic reaction in humans and animals. The aim of this study was to evaluate the ability of bleomycin to induce interstitial pulmonary fibrosis in mice.Material and methods: This study was conducted on C57BL/6 mice (14). Normal C57BL/6 mice (6) received 0.25 ml normal saline intraperotineal twice weekly for 8 weeks and sacrificed two days after last saline dose injection.8 mice received intraperotineal injection of 0.5mg bleomycin twice weekly for 8 weeks only 6 mice completed the study and sacrificed two days after last bleomycin dose. Weight recorded once weekly.Results: bleomycin induced fibrosis in the animal model after eight weeks with p<0.001Conclusion: bleomycin in a dose of 0.5mg for 8weeks induce pulmonary fibrosis in C57BL/6 MICE
Keywords: Bleomycin; intraperotineal injection; interstitial pulmonary fibrosis