EFFECTS OF BOTH GARLIC AND ROSEMARY EXTRACTS ON RATS LIVER FIBROSIS : A HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL STUDY

Abstract

Background: Liver fibrosis is a reversible scarring response that occurs in almost all patients with chronic liver injury.Liver fibrosis may progress to liver cirrhosis, the end-stage liver disease, which has been a globally increasing major health problem with high mortality and morbidity rates in the past twenty years.
Aim: This study aimed at detection of the effects of both Garlic and Rosemary extracts as natural sources on carbon tetrachloride (CCL4) induced rat liver fibrosis.
Materials and methods: This experimental study was carried out on 48 adult male albino rats that were classified into five groups(A-B-C-D-E) each group contain ten rats except group (B)contained eight rats.Group (A) is a control group did not receive any medication, group (B) received Carbon tetrachloride (CCL4) only,group (C) received (CCL4) and Garlic extract,group (D) received (CCL4) and Rosemary extract and group (E) received (CCL4) with both Garlic and Rosemary extracts. This work was carried out during the period from July 2013 to July 2014.
Results: Livers harvested from rats treated with Garlic extract and rats treated with Rosemary extract revealed marked reduction in both the inflammatory reactions and degenerative changes,as well as, reduction of fibrosis.Rats treated with both Garlic and Rosemary extracts revealed mild or niether reduction of inflammation nor fibrosis. microvesicular steatosis was noticed in Rosemary treated group (D).
Conclusion:Both Garlic and rosemary extracts administration with CCL4 significantly ameliorate all necroinflammatory and fibrotic changes in liver tissues when used individually.Thier protective effect was reduced when they were combined together .
Key words : Liver, Garlic, Rosemary, cirrhosis, fibrosis.